|
KMID : 0378019810240020041
|
|
New Medical Journal
1981 Volume.24 No. 2 p.41 ~ p.50
|
|
|
Experimental Studies on Thyroid Tissue Alloimplantation into Muscle, Peritoneum, and Portal Vein in Rabbits
|
|
|
|
|
Abstract
|
|
|
For the management of thyroid insufficiency induced by medical treatment with 1131 or near total surgical excision of gland for Graves´ disease and for suppression therapy in well differentiated thyroid cancer after total thyroidectomy, permanent oral medication is
mandatory.
With development and advancement of organ transplantation, transplantation of endocrine gland has been also attempted in various experimental animals and it has been proved cellular implantation of endocrine tissue is technically more feasible than whole organ transplantation with vascular anastomosis is.
The implanting sites of endocrine tissue and levels of physiologic requirement of hormone, "Halsted´s law of deficiency", are two significant factors to the viability and the maintenance of secretory function of isografting tissue, and rejection is one more significant factor in alloimplantation.
Many investigators reported that allograft has resulted in rejection unless the recipient was immunosuppressed. And so several methods, diffusion chamber technique, cell or tissue culture, milipore chamber technique and immunosuppressive drug, were tested for immunosuppression of rejection.
Recently it has been known that liver plays significant roles on suppressing and inactivating the transplantation antigen.
This experiment was carried out to establish adequate implanting site and the effect of immunosuppression for thyroid alloimplantation.
Implantation of the tissue into muscle, peritoneum, portal vein and immunosuppression by Imuran and Prednisolone were investigated.
The experimental animals were divided into 3 groups; alloimplantation into muscle, into peritoneum, into portal vein. Each group was divided immunosuppressed group and control group which was not immunosuppressed.
For the confirmation of viability and function of implanting cells, serum T4 & T3 value was checked from systemic vein and microscopic changes of implanting tissues were examined in all of the animals.
The results were as follows:
1. Hypothyroidism state was established between 14th - 21st day after total thyroidectomy in normal rabbits.
2. In all alloimplantation without immunosuppression, mean serum value of T4 and T3 were markdly decreased and microscopic findings showed disappearance of thyroid cells ,or follicles with marked lymphocyte infiltration and fibrosis at 2nd week after implantation.
3. In alloimplantation into muscle and peritoneum with immunosuppression mean serum value of T4 and T3 were slightly increased but below normal value and microscopic findings showed disappearance of thyroid follicles with lymphocyte infiltration and fibrosis at 3rd week after implantation.
4. In alloimplantation into portal vein, mean serum value of T4 and T3 were increased above normal value and microscopic findings showed well formed thyroid follicles and a little lymphocyte infiltration or fibrosis at 3rd week after implantation.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|